6-Hydroxy-D-nicotine oxidase of Arthrobacter oxidans. Gene structure of the flavoenzyme and its relationship to 6-hydroxy-L-nicotine oxidase

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Localization of the enantiozymes of 6-hydroxy-nicotine oxidase in Arthrobacter oxidans by electron immunochemistry.

During the course of growth of Arthrobacter oxidans, induction of the enantiozymes 6-hydroxy-D-nicotine oxidase and 6-hydroxy-L-nicotine oxidase occurred in the presence of DL-nicotine. Cryoultramicrotomed sections obtained from cells grown to stationary phase were gold immunolabeled. The results obtained demonstrate that both enzymes are localized in the cytoplasm.

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Binding of FAD to 6-hydroxy-D-nicotine oxidase apoenzyme prevents degradation of the holoenzyme.

Expression of the 6-hydroxy-D-nicotine oxidase (6-HDNO) gene from Arthrobacter oxidans cloned into Escherichia coli showed a marked temperature-dependence. Transformed E. coli cells grown at 30 degrees C exhibited a several-fold higher 6-HDNO activity than did cells grown at 37 degrees C. This effect did not depend on the promoter used for expression of the cloned gene in E. coli, nor was it an...

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Covalently bound flavin in D-6-hydroxynicotine oxidase from Arthrobacter oxidans. Purification and properties of D-6-hydroxynicotine oxidase.

oxidase 3 . Moreover, methylene blue was shown to be a strong inhibitor of the overall reaction. Oneelectron acceptors are also ineffective with the Dspecific enzyme; however, methylene blue and 2.6dichlorophenol-indophenol are able to reoxidize the reduced D-6-hydroxynicotine oxidase. In the presence of these dyes the rates of the overall process are higher than those with oxygen. Anaerobic ti...

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6-hydroxy-l-nicotine from Arthrobacter Nicotinovorans Facilitate Spatial Memory Formation in Rats

Effects of 6-hydroxy-L-nicotine derived from nicotine catabolism in Arthrobacter nicotinovorans on learning and memory processes were examined in adult male Wistar rats. 6-hydroxy-L-nicotine (0.3 mg/kg, i.p., 7 consecutive days chronic administration) significantly increased spontaneous alternation in Y-maze task and working memory in radial arm-maze task, suggesting effects on short-term memor...

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l-Hydroxy acid oxidase.

In previous communications (l-4) the properties and isolation of the l-amino acid oxidase of rat kidney were described. Quite accidentally, it was found that solutions of the enzyme were able to catalyze the oxidation of Z-hydroxy acids, and further study disclosed that this ability ran parallel with l-amino acid oxidase activity from the first crude extract to the final electrophoretically hom...

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ژورنال

عنوان ژورنال: European Journal of Biochemistry

سال: 1987

ISSN: 0014-2956,1432-1033

DOI: 10.1111/j.1432-1033.1987.tb13338.x